Quantitative estimation of irbesartan in two different matrices and its application to human and dog bioavailability studies using LCeMS/MS

作     者：Hari Krishan Tiwari Tausif Monif Priya Ranjan Prasad Verma Simrit Reyar Arshad Hussain Khuroo Sanjeev Mishra 

作者机构：Department of Clinical Pharmacology and PharmacokineticsRanbaxy Research LaboratoriesPlot:GP-5Sec-18HSIIDCOld Delhi-Gurgaon RoadGurgaon 122015HaryanaIndia Birla Institute of TechnologyRanchiJharkhandIndia 

出 版 物：《Asian Journal of Pharmaceutical Sciences》 (亚洲药物制剂科学（英文）)

年 卷 期：2013年第8卷第6期

页      面：346-355页

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：The authors acknowledge Ranbaxy Laboratories Limited to supports this study 

主　　题：Irbesartan Pharmacokinetics Solid phase extraction LCeMS/MS 

摘      要：A simple,rapid and robust high-throughput assay for the quantitative analysis of irbesartan in plasma from two species using liquid chromatography tandem mass spectrometric method was developed and *** phase extraction was used and quantification was achieved using a positive electrospray ionization interface under multiple reactions monitoring *** validation(as per recent regulatory requirements)was done using human *** same method was validated using dog plasma and the validation parameters met respective acceptance *** method has shorter run time of 3 *** will help in high *** low aliquot volume in the method helped us to take enough sampling time points for better pharmacokinetic *** calibration curve was shown to be linear from 12.1 to 6018.7 ng/ml in human plasma and 12.1 to 5987.2 ng/ml in dog *** method was successfully applied to samples collected during bioavailability studies of irbesartan in humans and dogs.