BACE1 in the retina:a sensitive biomarker for monitoring early pathological changes in Alzheimer's disease

作     者：Lan Li Jia Luo Dan Chen Jian-bin Tong Le-ping Zeng Yan-qun Cao Jian Xiang Xue-gang Luo Jing-ming Shi Hui Wang Ju-fang Huang 

作者机构：Department of Anatomy and NeurobiologySchool of Basic Medical SciencesCentral South University Department of AnesthesiologyThird Xiangya Hospital of Central South University Department of OphthalmologySecond Xiangya HospitalCentral South University 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2016年第11卷第3期

页      面：447-453页

核心收录：

学科分类：1002[医学-临床医学] 100203[医学-老年医学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(to JFH,DC,JBT),No.81371011,81400399,81471107 a grant from the Project of Innovation-driven Plan of Central South University(to DC),No.2015CXS022 a grant from the National Key Technologies Research and Development Program of China(to JFH),No.2012BAK14B03 Fundamental Research Funds of Central South University of China(to HW),No.2010QZZD022 Graduate Thesis Innovation Foundation of Central South University of China(to LL),No.2011ssxt106 

主　　题：nerve regeneration neurodegenerative disease Alzheimer's disease retina amyloid β β site amyloid precursor protein cleaving enzyme 1 APP/PS 1 transgenic mouse neural regeneration 

摘      要：Because of a lack of sensitive biomarkers,the diagnosis of Alzheimer＇s disease（AD） cannot be made prior to symptom ***,it is crucial to identify novel biomarkers for the presymptomatic diagnosis of *** brain lesions are a major feature of AD,retinal pathological changes also occur in *** this study,we investigated the temporal changes in β-site APP-cleaving enzyme 1（BACE1） expression in the retina and brain to determine whether it could serve as a suitable biomarker for early monitoring of ***/PS-1 transgenic mice,3,6 and 8 months of age,were used as an experimental group,and age-matched C57/BL6 wild-type mice served as the control *** the Morris water maze test,there were no significant differences in escape latency or in the number of crossings in the target area among mice of different *** with wild-type mice,no changes in learning or memory abilities were detected in transgenic mice at 3 months of ***,compared with wild-type mice,the escape latency was significantly increased in transgenic mice at 6 months,starting on day 3,and at 8 months,starting on day 2,during Morris water maze *** addition,the number of crossings of the target area was significantly decreased in transgenic *** learning and memory abilities of transgenic mice were further worsened at 8 months of *** staining revealed no BACE1 plaques in wild-type mice at 3,6 or 8 months or in transgenic mice at 3 months,but they were clearly found in the entorhinal cortex,hippocampus and prefrontal cortex of transgenic mice at 6 and 8 ***1 expression was not detected in the retina of wild-type mice at 3 months,but weak BACE1 expression was detected in the ganglion cell layer,inner plexiform layer and outer plexiform layer at 6 and 8 *** transgenic mice,BACE1 expression in the ganglion cell layer was increased at 3 months,and BACE1 expression in the ganglion cell layer,inner plexiform layer and outer plex