Effect of cholecystokinin and secretin on contractile activity of isolated gastric muscle strips in guinea pigs

作     者：Li W Zheng TZ Qu SY 

作者机构：Department of PhysiologyLanzhou Medical CollegeLanzhou 730000Gansu ProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2000年第6卷第1期

页      面：93-95页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：the Natural Science Foundation of Gansu Province No.ZR-94-085 

主　　题：Subject headings gastric muscle strips cholecystokinin secretin guinea pigs 

摘      要：AIM To study the effect of cholecystokinin-octapeptide（CCK-8）and secretin on contractileactivity of isolated gastric muscle strips inguinea *** Each isolated gastric muscle stripwas suspended in a tissue chamber containing5 mL Krebs solution constantly warmed by waterjacked at 37℃ and supplied with a mixed gas of95% O2 and 5% CO2 After incubating for lhunder 1 g tension,varied concentrations of CCK-8 and secretin were added respectively in thetissue chamber and the contractile response wasmeasured isometrically on ink-writing *** CCK-8 could increase①all regionalcircular and longitudinal muscular tension at rest（fundus LM 19.7%±2.1%,P0.01;fundus CM16.7%±2.2%,P0.01;gastric body LM 16.8%±2.3%,P0.01;body CM 12.7%±2.6%,P0.01;antrum LM 12.3%±1.3%,P0.01;antrum CM 16.7%±4.5%,P0.01;pylous CM12.7%±5.0%,P0.05）;②contractilefrequencies of body LM,both LM and CM ofantrum and pylorus CM（5.1/min±0.2/min to5.6/min±0.2/min,5.9/min±0.2/min to 6.6/min±0.l/min,5.4/min±0.3/min to 6.3/min±0.4/rain,1.3/min±0.2/min to 2.3/min±0.3/min,respectively,P0.05）;③the mean contractileamplitude of antral circular muscle（58.6%±18.4%,P0.05）and ④the motility index ofpylorus CM（145.0%±23.8%,P0.01）,butdecrease the mean contractile amplitude ofgastric body and antral LM（-10.3%±3.3%,-10.5%±4.6%,respectively,P0.05）.All the CCK-8 effects were not blocked by atropine *** had no effect on gastricsmooth muscle *** CCK-8 possessed bothexcitatory and inhibitory action on contractileactivity of different regions of stomach in *** action was not mediated via cholinergicM receptor and endogenous prostagiandinreceptor,