Expression of matrix metallo-proteinase-28 in human normal cytotrophoblast cells and a choriocarcinoma cell line,JEG-3

作     者：Zhang, J Cao, YJ Liu, WM Zhao, BS Zeng, GQ Duan, EK 

作者机构：Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第9期

页      面：732-736页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by the Special Funds for the Major State Basic Research Project (Grant No. G1999055903) the Climbing Project of China (Grant No. 970211019-3) the Hundred-Scientist-Program of the Chinese Academy of Sciences 

主　　题：cytotrophoblast cell choriocarcinoma cell line JEG-3 matrix metalloproteinases MMP-28. 

摘      要：There are striking similiarities present between the behavior of invasive placentaL cells and that of invasive cancer cells. Matrix metalloproteinases (MMPs) are one of the most important mediators. MMP-28, the new member of MMPs, was sequenced and identified recently. Expression of MMP-28 mRNA and protein in the cytotrophoblast cells and a choriocarcinoma cell line, JEG-3 cell, was conducted by zymography, RT-PCR and Northern blot. There is MMP-28 mRNA expression in both the cytotrophoblast cells and JEG-3 cells by RT-PCR. The activity of MMP-28 in cytotrophoblast cells was significantly weaker than that in JEG-3 (P 0.01) by zymography. Furthermore, mRNA expression of MMP-28 was significantly stronger (P 0.001) in JEG-3 than in human cytotrophoblast cells in a time-dependent way by Northern Blot. Our results suggest that MMP-28 may play a role in some of the tissue-remodeling events associated with normal pregnancy and tumor progression.