Investigation of the binding behavior of human serum albumin and phosphorothioate oligodeoxynucleotide

作     者：叶国柱 张亮仁 张礼和 Guo-Zhu Ye;Liang-Ren Zhang;Li-He Zhang

作者机构：北京大学药学院天然药物及仿生药物国家重点实验室北京100083 

出 版 物：《Journal of Chinese Pharmaceutical Sciences》 (中国药学（英文版）)

年 卷 期：2007年第16卷第1期

页      面：9-13页

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China(20472007) 

主　　题：Phosphoruthioate oligodeoxynucleotide Human serum albumin Positive potential region Conformational changes 

摘      要：Aim To study the binding behavior between human serum albumin （HSA） and phosphorothioate oligodeoxynucleotide （PS- ODN） and the effects of bivalent cations on the interaction. Methods Surface plasma resonance, circular dichroism and fluorescence experiments were conducted. Results （ 1 ） the binding ability was decreased along with the increase of pH; （2） Zn^2＋and Ni^2＋ enhanced the interaction between PS-ODN and HSA; （3） Upon PS-ODN binding, the conformation of HSA was changed with an increase of β - sheet. Conclusion The results provide experimental evidences to the hypothesis that PS-ODN binds with HSA in the positive potential region, and histidine residues located in the region play a crucial rule in the interaction.