Expression of recombinant rat Neurotrophin-3 in Chinese hamster ovary cells
Expression of recombinant rat Neurotrophin-3 in Chinese hamster ovary cells出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)
年 卷 期：1999年第42卷第6期
页 面：655-662页
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
基 金：Project supported by the Natural Science Foudation of Guangdong Province China
主 题：Neurotrophin-3 cDNA recombinant nerve fiber outgrowth.
摘 要：The CHO cell line stably producing recombinant rat NT-3 was established. The insertion of rNT-3 cDNA into transferred cell gonome was analyzed with Southern blot. The expressed protein was identified by Dot ELISA (enzyme-linked immunosorbent assay) and Western blot. Western blot showed a clear specific band of about 14 ku for NT-3. The mean level of rNT-3 in four NT-3cDNA/CHO cell lines was about 2 100 ng/106 cells/48 h determined by EIA. The conditioned-medium (CM) of NT-3cDNA/CHO cells could promote the fiber outgrowth of the dissociated dorsal root ganglion of 8-day-old chick embryos, which shows a dose-response relationship. A half-maximal concentration of the biological activity (EC50) of the recombinant protein was approximately 16.7 ng/mL. The MoAb 3W3 of NT-3 could neutralize the biological activity of the rNT-3.
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