Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis
作者机构：School of Public HealthResearch Center of Public HealthRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China Department of Radiation and Medical OncologyZhongnan Hospital of Wuhan UniversityWuhan UniversityWuhan 430071China Key Laboratory of Magnetic Resonance in Biological SystemsState Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of Sciences-Wuhan National Laboratory for OptoelectronicsWuhan 430071China University of Chinese Academy of SciencesBeijing 101408China Institute of Neuroscience and Brain DiseasesXiangyang Central HospitalAffiliated Hospital of Hubei University of Arts and ScienceXiangyang 441000China Phase I Clinical Trial LaboratoryZhongnan Hospital of Wuhan UniversityWuhan 430071China
出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)
年 卷 期：2024年第35卷第8期
页 面：413-417页
核心收录：
学科分类：1002[医学-临床医学] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by the National Key R&D Program of China(Nos.2022YFC3400700,2022YFA0806600) the National Natural Science Foundation of China(Nos.22277093,22074110,21721005) the Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(No.JCRCGW-2022-008) the Wuhan Knowledge Innovation Project(No.2022020801010111) the Natural Science Foundation of Hubei Province(No.2022CFB569)
主 题：Sleep deprivation Epigenetics Mass spectrometry DNA modification RNA modification Small RNA
摘 要：Sleep deprivation(SD)is a widespread issue that disrupts the lives of millions of *** effects ini-tiate as changes within neurons,specifically at the DNA and RNA level,leading to disruptions in neuronal plasticity and the dysregulation of various cognitive functions,such as learning and *** acid epigenetic modifications that could regulate gene expression have been reported to play crucial roles in this ***,there is a lack of comprehensive research on the correlation of SD with nucleic acid epigenetic *** the current study,we aimed to systematically investigate the landscape of modifications in DNA as well as in small RNA molecules across multiple tissues,including the heart,liver,kidney,lung,hippocampus,and spleen,in response to chronic sleep deprivation(CSD).Using liquid chromatography-tandem mass spectrometry(LC-MS/MS)analysis,we characterized the dynamic changes in DNA and RNA modification profiles in different tissues of mice under CSD ***,we ob-served a significant decrease in the level of 5-methylcytosine(5mC)and a significant increase in the level of 5-hydroxymethylcytosine(5hmC)in the kidney in CSD *** RNA modifications,we observed an overall increased trend for most of these significantly changed modifications across six tis-sues in CSD *** study sheds light on the significance of DNA and RNA modifications as crucial epigenetic markers in the context of CSD-induced stress.
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