Mutation induction of Pleurotus ferulae by low-energy N^+ ion implantation and characters of the selected mutant

作     者：CHEN Henglei WAN Honggui ZHANG Jun ZENG Xianxian 

作者机构：College of Life Science and Pharmacy Engineering Nanjing University of Technology Nanjing 210009 China Ion Beam Bio-engineering Center Xinjiang University Urumchi 830008 China 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2008年第19卷第1期

页      面：13-16页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by the National Developing and Reform Committee Project of China 2077 

主　　题：突变原理 侧耳 高温环境 辐射损伤 

摘      要：In order to obtain Pleurotus ferulae with high temperature tolerance, mycelium mono-cells of wild type strain ACK was treated by nitrogen ion (5~30 keV, 1.5×1015~1.5×1016 cm-2) implantation, and mutant CGMCC1762 was selected through auxotrophy screening method, which was Lys, VB6 auxotrophy stress with high temperature. We found that during riper period the surface layer mycelium of the mutant was not aging neither grew tegument even above 30°C. The mycelium endurable temperature of the mutant was increased 7°C compared with that of the wild type strain. The fruiting bodies growth temperature of the mutant was 16~20°C in daytime and was 6~12°C at night. The highest growth temperature of fruiting bodies of the mutant was increased by 5°C than that of original strain. Through three generation investigation, we found that the mutant CGMCC1762 was stable with high temperature tolerance.