Comparative metabolomics reveal histamine biodegradation mechanism by salt stressed Bacillus subtilis JZXJ-7
作者机构：College of Food Science and Engineering， Bohai University Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing Department of Wine， Food and Molecular Biosciences， Faculty of Agriculture and Life Sciences， Lincoln UniversityLincoln 7647， New Zealand College of Food Science， Southwest University College of Food Science and Engineering， Lingnan Normal University
出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康(英文))
年 卷 期：2024年
核心收录：
学科分类：08[工学] 082203[工学-发酵工程] 0822[工学-轻工技术与工程]
基 金：supported by the National Key R&D Program of China (2019YFD0901702) National Natural Science Foundation of China (32202134 32201929) China Postdoctoral Science Foundation (2022M712625) 2021 and 2022 Chongqing Postdoctoral Special Funding Project
摘 要：Bacillus subtilis (B. subtilis) JZXJ-7 isolated from shrimp paste can significantly degrade histamine under salt stress but the mechanism is unclear. This study aims to evaluate the effect of 170 and 340 mmol/L NaCl on B. subtilis JZXJ-7 growth， histamine degradation， antioxidant enzymes [catalase (CAT)， superoxide dismutase (SOD) and glutathione S-transferase (GST)] activities and Na+， K+-ATPase activity. Furthermore， comparative metabolomics was used to investigate histamine biodegradation mechanism by *** JZXJ-7 subjected to salt stress. Both 170 and 340 mmol/L NaCl promoted B. subtilis JZXJ-7 growth in late stages of reproduction (32 - 48 h)， increased histamine degradation rate by 64.85% and 79.87% (P 0.05)， Na+， K+-ATPase activity to 6.28 U/mg (P 0.05) and 11.63 U/mg (P 0.01) respectively. NaCl treatment significantly increased the activities of CAT， GST and SOD (P 0.05)， amino acids and its metabolites (33.39%)， benzene and substituted derivatives (12.05%)， heterocyclic compounds (10.62%)， organic acids and derivatives (9.75%)， aldehydes， ketones， esters (5.59%) and nucleotides and its metabolites (4.58%). Metabolite set enrichment analysis revealed NaCl induced differential metabolic pathways of D-glutamine， D-glutamate， L-arginine， L-proline， histidine and glycerophospholipids， L-lysine degradation， and aminoacyl-tRNA biosynthesis. Exposure to 340 mmol/L NaCl up-regulated carbohydrate， glutathione and glycerophospholipid metabolism. The new insights into the mechanism of salt stress to promote B. subtilis JZJX-7 growth， energy metabolic pathways and to degrade histamine provide the theoretical basis for application of B. subtilis JZXJ-7 in food fermentation industry.
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