一种miRNA标记在胶质瘤侵袭性表型的确定与预后评估中的价值(英文)

作     者：Barbano R Palumbo O Pasculli B Galasso M Volinia S D'Angelo V Icolaro N Coco M Dimitri L Graziano P Copetti M Valori VM Maiello E Carella M Fazio VM Parrella P 

作者机构：Laboratory of Oncology"IRCCS Casa Sollievo della Sofferenza" Laboratory of Genetics"IRCCS Casa Sollievo della Sofferenza" Department of BiosciencesBiotechnology and BiopharmaceuticsUniversity of Bari "Aldo Moro" LTTADepartment of MorphologySurgery and Experimental MedicineHuman Anatomy BranchUniversity of Ferrara Department of Neurosurgery"IRCCS Casa Sollievo della Sofferenza " Department of Pathology"IRCCS Casa Sollievo della Sofferenza" Unit of Biostatistics"IRCCS Casa Sollievo della Sofferenza" Department of Oncology"IRCCS Casa Sollievo della Sofferenza" CIR Laboratory for Molecular Medicine and BiotechnologyUniversity Campus Biomedico 

出 版 物：《中华神经外科疾病研究杂志》 (Chinese Journal of Neurosurgical Disease Research)

年 卷 期：2014年第13卷第5期

页      面：433-433页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：神经外科学 疾病 临床研究 医学 

摘      要：Gliomas represent a disparate group of tumours for which there are to date no cure. Thus,there is a recognized need for new diagnostic and therapeutic approaches based on increased understanding of their molecular nature. We performed the comparison of the microRNA( miRNA) profile of 8 WHO grade II gliomas and 24 higher grade tumours( 2 WHO grade III and 22 glioblastomas) by using the Affymetrix Gene Chip miRNA Array v. 1. 0. A relative quantification method( RT-q PCR) with standard curve was used to confirm the 22 miRNA signature resulted by array analysis. The prognostic performances of the confirmed miRNAs were estimated on the Tumor Cancer Genome Atlas( TCGA) datasets. We identified 22 miRNAs distinguishing grade II gliomas from higher grade ***-q PCR confirmed the differential expression in the two patients groups for 13 out of the 22 miRNAs. The analysis of the Glioblastoma Multiforme( GBM) and Lower Grade Glioma( LGG) datasets from TCGA demonstrated the association with prognosis for 6 of those miRNAs. Moreover,in the GBM dataset miR-21 and miR-210 were predictors of worse prognosis in both univariable and multivariable Cox regression analyses( HR 1. 19,P = 0. 04,and HR 1. 18,P = 0. 029 respectively). Our results support a direct contribution of miRNAs to glioma cancerogenesis and suggest that miR-21 and miR-210 may play a role in the aggressive clinical behaviour of glioblastomas.