Construction of DNA Fingerprint Using SSR Marker for Hybrid Rice Cultivars Approved by Hunan Province

作     者：Qingcai ZHAN Zifa XIAO Keyong ZHU Zhixi LIU Zhaohua LI Shuzhen ZENG 

作者机构：Laboratory of BiotechnologyHunan Rice Research Institute Graduate School of Central South UniversityLongping Branch 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2012年第1卷第4期

页      面：7-10,14页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Supported by Agricultural Science and Technology Achievements Transformation Fund of Ministry of Science and Technology (2007GB2D200225) Master Degree Paper Innovation Fund of Central South University (2010SSXt045) 

主　　题：Hybrid rice SSR marker Fingerprint map Germplasm identification 

摘      要：[Objective] This study aimed to construct DNA fingerprint for hybrid rice cultivars those have been approved by Hunan Province. [Method] The primers which produced polymorphic and bright DNA bands were selected to construct the DNA molecular fingerprint map for 77 major hybrid rice cultivars approved by Hunan Province. [ Result] A total of 48 SSR primers were selected. Every cultivar had its unique fingerprint map so that the obtained data could identify differ- ent hybrid rice cultivars. [ Conclusion] This study made great contributions to the perfection of hybrid rice germplasm identification.