Rapid Multi-Wavelength Optical Assessment of Circulating Blood Volume Without a Priori Data

作     者：Ekaterina V. LOGINOVA Tatyana V. ZHIDKOVA Mikhail A. PROSKURNIN Vladimir E ZHAROV 

作者机构：M. V. Lomonosov Moscow State University Chemistry Department Moscow 119991 Russia Agilent Technologies Partner Laboratory / M.V. Lomonosov Moscow State University Analytical Center Moscow 119991 Russia philips Classic Laser Laboratories University of Arkansas for Medical Sciences Little Rock Arkansas 72205 USA 

出 版 物：《Photonic Sensors》 (光子传感器（英文版）)

年 卷 期：2016年第6卷第1期

页      面：42-57页

核心收录：

学科分类：12[管理学] 082803[工学-农业生物环境与能源工程] 0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 1204[管理学-公共管理] 082304[工学-载运工具运用工程] 0828[工学-农业工程] 080204[工学-车辆工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 120405[管理学-土地资源管理] 0702[理学-物理学] 0823[工学-交通运输工程] 

基　　金：The work is supported by the Russian Science Foundation  grant no. 14-23-00012. We are grateful to Agilent Technologies - Russia and its CEO  Dr. Konstantin Evdokimov  for Agilent equipment used in this study 

主　　题：Circulating blood volume assessment spectrophotometry methylene blue crystal violet 

摘      要：The measurement of circulating blood volume （CBV） is crucial in various medical conditions including surgery, iatrogenic problems, rapid fluid administration, transfusion of red blood ceils, or trauma with extensive blood loss including battlefield injuries and other emergencies. Currently, available commercial techniques are invasive and time-consuming for trauma situations. Recently, we have proposed high-speed multi-wavelength photoacoustic/photothermal （PA/PT） flow cytometry for in vivo CBV assessment with multiple dyes as PA contrast agents （labels）. As the first step, we have characterized the capability of this technique to monitor the clearance of three dyes （indocyanine green, methylene blue, and trypan blue） in an animal model. However, there are strong demands on improvements in PA/PT flow cytometry. As additional verification of our proof-of-concept of this technique, we performed optical photometric CBV measurements in vitro. Three label dyes--methylene blue, crystal violet simultaneous photometric determination of the and, partially, brilliant green--were selected for components of their two-dye mixtures in the circulating blood in vitro without any extra data （like hemoglobin absorption） known a priori. The tests of single dyes and their mixtures in a flow system simulating a blood transfusion system showed a negligible difference between the sensitivities of the determination of these dyes under batch and flow conditions. For individual dyes, the limits of detection of 3×10-6M-3×10-5M in blood were achieved, which provided their continuous determination at a level of 10-SM for the CBV assessment without a priori data on the matrix. The CBV assessment with errors no higher than 4% were obtained, and the possibility to apply the developed procedure for optical photometric （flow cytometry） with laser sources was shown.