16S rRNA is a better choice than COI for DNA barcoding hydrozoans in the coastal waters of China

作     者：ZHENG Lianming HE Jinru LIN Yuanshao CAO Wenqing ZHANG Wenjing 

作者机构：College of Ocean and Earth Sciences Xiamen University Marine Biodiversity and Global Change Research Center Xiamen University Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies (CEES) XiamenUniversity 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2014年第33卷第4期

页      面：55-76页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0908[农学-水产] 0707[理学-海洋科学] 071007[理学-遗传学] 0713[理学-生态学] 

基　　金：The National Natural Science Foundation of China under contract No.41006078 the Fundamental Research Funds for the Central Universities under contract No.2010121037 the Public Science and Technology Research Funds Projects of Ocean under contract Nos 201005012-3 and 201005015-5 the Natural Science Foundation of Fujian Province of China under contract No.2011J05116 

主　　题：DNA barcoding hydrozoan COI 16S rRNA 

摘      要：Identification of hydrozoan species is challenging, even for taxonomic experts, due to the scarcity of distinct morphological characters and phenotypic plasticity. DNA barcoding provides an efficient method for species identification, however, the choice between mitochondrial cytochrome c oxidase subunit I（COI） and large subunit ribosomal RNA gene（16S） as a standard barcode for hydrozoans is subject to debate. Herein, we directly compared the barcode potential of COI and 16S in hydrozoans using 339 sequences from 47 pelagic hydrozoan species. Analysis of Kimura 2-parameter genetic distances（K2P） documented the mean intraspecific/interspecific variation for COI and 16S to be 0.004/0.204 and 0.003/0.223, respectively. An obvious ＂barcoding gap＂ was detected for all species in both markers and all individuals of a species clustered together in both the COI and 16S trees. These results suggested that the species within the studied taxa can be efficiently and accurately identified by COI and 16S. Furthermore, our results confirmed that 16S was a better phylogenetic marker for hydrozoans at the genus level, and in some cases at the family level. Considering the resolution and effectiveness for barcoding and phylogenetic analyses of Hydrozoa, we strongly recommend 16S as the standard barcode for hydrozoans.