Study on the Therapeutic Effects and Mechanisms of Human Mesenchymal Stem Cell-Derived Exosomes Carrying NGF Gene in Treating Ischemic Stroke in Rats

作     者：Bingqian Li Xuanxuan Xu Wenqin Zhou Peng Wang 

作者机构：Affiliated People’s Hospital of Jiangsu UniversityZhenjiang 212000Jiangsu ProvinceChina 

出 版 物：《Proceedings of Anticancer Research》 (抗癌研究)

年 卷 期：2024年第8卷第4期

页      面：41-47页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Clinical Medicine Science and Technology Development Fund of Jiangsu University 2021(Natural Science Category)(Grant No.JLY2021004) 

主　　题：NGF gene Human mesenchymal stem cell-derived exosomes Ischemic stroke in rats Mechanism of action 

摘      要：Objective:To investigate the therapeutic effects and mechanisms of human mesenchymal stem cell-derived exosomes(hMSCs-Exo)carrying the NGF gene in treating ischemic stroke in rats,aiming to provide new insights and treatment methods for ischemic stroke ***:After successful construction of the cerebral ischemia model in 40 male SPF-grade SD rats aged 6-8 weeks,the model rats were randomly divided into 4 groups:Sham group,PBS group,hMSCs-Exo group,and NGF-hMSCs-Exo group,with 10 rats in each *** rat MCAO model was prepared using the classic filament method,and NGF-hMSCs-Exo were injected via the tail vein into the MCAO model *** expression of the NGF gene in brain ischemic tissues,neuronal regeneration,and rat neurological function recovery were observed using TTC staining,memory function evaluation,Western blot,qRT-PCR,and other ***:Compared with the Sham group,neurological deficits were significant in the PBS group(P0.01).Compared with the PBS group,neurological scores improved in the hMSCs-Exo group and NGF-hMSCs-Exo group(P0.05).Compared with the hMSCs-Exo group,the improvement in neurological deficits was more significant in the NGF-hMSCs-Exo group(P0.05).The infarct area after NGF-hMSCs-Exo intervention was significantly reduced(P0.05)compared with the Sham *** with the PBS group,relative expression levels of NGF mRNA and protein decreased,while Caspase-3 mRNA and protein expression significantly increased in the PBS group(P0.01).Compared with the PBS group and hMSCs-Exo group,there were differences in NGF and Caspase-3 mRNA and protein expression in the NGF-hMSCs-Exo group rat brain tissues(P0.05).Conclusion:Treatment with human mesenchymal stem cell-derived exosomes carrying the NGF gene improves cognitive function and exerts protective effects on SD rats while inhibiting apoptotic levels in cells.