Light sheet fluorescence microscopy: Advancing biological discovery with more dimensions, higher speed, and lower phototoxicity

作     者：Yao Zhou Shiqi Mao Peng Fei 

作者机构：School of Optical and Electronic Information-Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China Advanced Biomedical Imaging FacilityHuazhong University of Science and TechnologyWuhan 430074China 

出 版 物：《The Innovation》 (创新（英文）)

年 卷 期：2024年第5卷第5期

页      面：29-30页

核心收录：

学科分类：080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0803[工学-光学工程] 

基　　金：supported by the National Key Research and Development Program of China(2022YFC3401102) the National Natural Science Foundation of China(T2225014 and 21927802) the Fundamental Research Funds for the Central Universities(YCJJ20242110) the Postdoctoral Fellowship Program of CPSF under grant number GZB20240246 

主　　题：fluorescence dimensions Figure 

摘      要：TO SEE THE WORLD IN A GRAIN OF *** microscopy has proved to be essential for seeing life in a grain of a *** epifluorescence microscopes,such as wide-field microscopes and laser scanning confocal microscopes,have been widely used for biology research over the past few *** goal of fluorescence microscopy is to observe the structure and function of cellular events across time and threedimensional(3D)space with spatiotemporal resolution as high as ***,conventional epifluorescence microscopes illuminate the entire 3D volume of the sample while detecting signals only from the 2D focal plane,thereby leading to low photon utilization and high phototoxicity issues that notably prevent high-resolution imaging of live biological specimens(Figure 1A).It becomes increasingly noteworthy that the massively emerging biomedical applications are posing significant challenges to current fluorescence microscopy techniques.