Accuracy Enhancement of the Folin-Ciocalteu Method for Propolis
Accuracy Enhancement of the Folin-Ciocalteu Method for Propolis作者机构：Instituto Federal de Educao Cincia e Tecnologia de Minas GeraisCampus Formiga Formiga MG Brazil Departamento de Qumica Instituto de Cincias Exatas Universidade Federal de Minas Gerais Belo Horizonte MG Brazil
出 版 物：《American Journal of Analytical Chemistry》 (美国分析化学（英文）)
年 卷 期：2024年第15卷第8期
页 面：253-267页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
主 题：Folin-Ciocalteu Multivariate Optimization Propolis Total Phenol
摘 要：This study presents an optimization of the Folin-Ciocalteu spectrophotometric method for the determination of total phenol content. Multivariate optimization using factorial planning 22 with a central point and central composite planning was constructed to evaluate the influence of variables in the process and maximize radiation absorption with minimal radiation scattering caused by solid formation. X-ray fluorescence and X-ray diffraction spectrometry were used to evaluate the chemical composition of solids formed and nephelometric and spectrophotometric studies were also used to evaluate whether the type, origin, dilution and dry extract contents of commercial propolis extracts would significantly influence the increase in radiation scattering and absorption. The optimized methodology added several advantages, such as reduction of reagents, time analysis, and higher accuracy.
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