Phosphoester modified poly(ethylenimine) as efficient and low cytotoxic genevectors

作     者：DU JinZhi TANG LingYan YUAN YouYong WANG Jun 

作者机构：Department of Polymer Science and Engineering CAS Key Laboratory of Soft Matter Chemistry University of Science and Technology of China Hefei 230026 China Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei 230027 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2011年第54卷第2期

页      面：351-358页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 070303[理学-有机化学] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Basic Research Program of China (973 Program 2010CB934001) 

主　　题：gene delivery PEI phosphoester gene vector 

摘      要：Cyclic phosphoester monomer ethyl ethylene phosphate (EEP) modified poly(ethylenimine) (PEI),denoted as PEI-EEP,was developed for gene *** PEI-EEP polymers were synthesized and their structures were characterized by 1H and 31P NMR *** the PEI-EEP polymers could condense DNA efficiently at N/P ratios higher than 0.5/*** physiochemical characteristics of PEI-EEP/DNA complexes were analyzed by particle size and zeta potential *** particle sizes of complexes were around 160–250 nm,and their zeta potentials were around 30–45 mV at the N/P ratios ranging from 10/1 to 50/*** vitro cell viability and transfection ability were evaluated in HEK293 and HeLa cells using PEI as the *** cytotoxicity of PEI-EEP and PEI-EEP/DNA complexes was lower than that of PEI and its complexes with *** transfection efficiency of PEI-EEP/DNA complexes was correlated to modification degrees with *** the modification of phosphoester to PEI was moderate,the PEI-EEP1/DNA and PEI-EEP2/DNA complexes exhibited comparable or even higher transfection ability than PEI/DNA complex at its optimal N/P ratio in the absence of ***,transfection efficiency of PEI-EEP3 reduced *** importantly,the PEI-EEP exhibited higher transfection efficiency in the presence of 10% serum than that without ***,PEI-EEP polymers may be attractive vectors for non-viral gene therapy.