Science Letters:Determination of benzalkonium chloride in viscous ophthalmic drops of azithromycin by high-performance liquid chromatography

作     者：Yan SHEN Sheng-jie XU Shi-chun WANG Jia-sheng TU 

作者机构：Department of Pharmaceutics China Pharmaceutical University Nanjing 210009 China Productivity Center of Jiangsu Province Nanjing 210042 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2009年第10卷第12期

页      面：877-882页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 070302[理学-分析化学] 0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 0817[工学-化学工程与技术] 0906[农学-兽医学] 0703[理学-化学] 10[医学] 

基　　金：Project (No. 2008BAI55B03) supported by the Key Project of the National Science and Technology Pillar Program  China 

主　　题：Benzalkonium chloride High-performance liquid chromatography Azithromycin Viscous ophthalmic drops 

摘      要：A high-performance liquid chromatography （HPLC） system was used in the reversed phase mode for the determination ofbenzalkonium chloride （BKC） in azithromycin viscous ophthalmic drops. A Venusil-XBP（L）-C18 （150 mm×4.6 mm, 5 gm） column was used at 50℃. The mobile phase consisted of a mixture of methanol-potassium phosphate （16：5, v/v）. Two sample preparation methods were compared. The results suggested that, compared with an extraction procedure, a deproteinization procedure was much quicker and more convenient. Using the deproteinization procedure for sample preparation, calibration curves were linear in the range 5.0-50μg/ml. The within-day and inter-day coefficients of variation were less than 10%. The average recoveries were determined as 96.70%, 98.52%, and 97.96% at concentrations of 10.0, 30.0, and 50.0 μg/ml, respectively. Variability in precision did not exceed 5%. In conclusion, this HPLC method using a simple sample treatment procedure appears suitable for monitoring BKC content in azithromycin viscous ophthalmic drops.