Characterization of toxin from Verocytotoxigenic Ecscherichia coli(VTEC) strains isolated from neonatal calves in India

作     者：Diganta Pan Ashok Kumar Bhatia Bhilegaonkar KN 

作者机构：Department of Microbiology and ImmunologyCollege of Veterinary Science and Animal HusbandryMathura-281001UP(India) Indian Veterinary Research InstituteIzatnagarU.P.243122India 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2009年第2卷第2期

页      面：35-38页

核心收录：

学科分类：090601[农学-基础兽医学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 09[农学] 0906[农学-兽医学] 

主　　题：Escherichia coli Non-O157 verocytotoxic Escherichia coli Verocell cytotoxicity assay Mice lethality test 

摘      要：Objective:The present study has characterized dialyzed toxin from non-0157 VTEC *** isolates by vero cell toxicity assay and pathogenecity in mice ***:Toxins from non-0157 verocytotoxic Escherichia coli isolated from neonatal calves were *** toxin from *** 026,0111 and 0103 serotypes were prepared and characterized by verocell toxicity assay and pathogenicity in mice *** 0157:H7 considered as positive control for this ***:Cytopathic effects in vero cell line first rounding of vero cells,followed by clumping of cells and finally disintegrated,blackened,shriveled cell line within 16 to 72 *** markers such as hind limb paralysis and reddening of tail were prominent in all the toxicated *** histopathological study was conducted for multiple organ ***: Several methods for toxin assay were developed based on biological,immunological and detection of virulence genes related to toxin production but each test has draw ***,it is likely that future effort will be focused on the development of assay,which is fast,reliable,specific and sensitive methods based on mice model.