Optimizing the Bacillus thuringiensis(Bt)protein concentration in cotton:Coordinated application of exogenous amino acids and EDTA to reduce spatiotemporal variability in boll and leaf toxins

作     者：Zhenyu Liu Shu Dong Yuting Liu Hanjia Li Fuqin Zhou Junfeng Ding Zixu Zhao Yinglong Chen Xiang Zhang Yuan Chen Dehua Chen 

作者机构：Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of GrainCropsYangzhou UniversityYangzhou 225009China The UWA Institute of AgricultureSchool of Agriculture and EnvironmentThe University of Western AustraliaPerth 6009WAAustralia 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第10期

页      面：3419-3436页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Natural Science Foundation of China(31901462 and 31671613) the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(22KJA210005) the China Scholarship Council(202308320440) the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX22_3508) 

主　　题：Gossypium hirsutum Bt cotton insecticidal protein bolls and their subtending leaves nitrogen metabolism 

摘      要：During the boll formation stage,cotton bolls exhibit the lowest expression of Bacillus thuringiensis(Bt)insecticidal *** to insects varies notably among different organs,which poses challenges for controlling cotton ***,an experimental strategy was designed in the 2020-2021 cotton growing season to coordinate the enhancement of protein synthesis and the attenuation of *** Bt cultivars of Gossypium hirsutum,namely the hybrid Sikang 3 and the conventional Sikang 1,were used as test *** treatments were applied at the peak flowering period:CK(the control),T1(amino acids),and T2(amino acids and EDTA).The results show that,in comparison to the CK group,the Bt protein contents were significantly increased in both cotton bolls and their subtending leaves under the T1 and T2 *** maximum levels of increase observed were 67.5%in cotton bolls and 21.7%in ***,the disparity in Bt protein content between cotton bolls and their subtending leaves notably decreased by 31.2%.Correlation analysis suggested that the primary physiological mechanisms for augmenting Bt protein content involve increased protein synthesis and reduced protein catabolism,which are independent of Bt gene expression *** regression and path analysis revealed that elevating the soluble protein content and transaminase activity,while reducing the catabolic enzyme activities,are instrumental in enhancing the Bt protein ***,the coordinated application of amino acids and EDTA emerges as a strategy that can improve the overall resistance of Bt cotton and mitigate the spatiotemporal variations in Bt toxin concentrations in both cotton bolls and leaves.