Mixed-charge glycopolypeptides as antibacterial coatings with long-term activity

作     者：Fangping Yang Jin Shi Yuansong Wei Qing Gao Jingrui Shen Lichen Yin Haoyu Tang 

作者机构：Institute of Functional Nano & Soft Materials(FUNSOM)Collaborative Innovation Center of Suzhou Nano Science and TechnologySoochow University 

出 版 物：《Chinese Chemical Letters》 (中国化学快报(英文版))

年 卷 期：2025年第36卷第2期

页      面：270-278页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 10[医学] 

基　　金：the financial support from the National Natural Science Foundation of China (No. 51873213) Science and Technology Program of Suzhou (No. SKY2022111) Collaborative Innovation Center of Suzhou Nano Science & Technology, and FUNSOM Self-Directed Research Project (No. 2022) 

主　　题：Antibacterial coating Bactericidal activity Biofilm Fouling Glycopolypeptide 

摘      要：Planktonic bacteria adhere and subsequently form biofilms on implantable medical devices can cause severe infections that have become the major types of hospital-acquired infections. Traditional coatings for the implants are frequently lack of long-term antifouling and bactericidal activities. It is still a big challenge to simultaneously improve the antifouling and bactericidal activities of the coatings. Herein,we report that mixed-charge glycopolypeptide coatings are of long-term antibacterial activities to efficiently inhibit the biofilm growth. The glycosylation of mixed-charge polypeptides has led to a significant improvement of both antifouling and bactericidal activities. The cooperative effect of the saccharide residues and mixed-charge residues improved the resistance of the polypeptide coatings against protein adsorption. The saccharide and L-glutamic acid(E) residues collectively enhanced the bacterial membrane-disruption of cationic L-lysine(K) residues, leading to potent bactericidal activity. Meanwhile,the glycopolypeptide coatings showed superior biocompatibility, long-term antibiofilm and anti-infection properties in two types of mouse subcutaneous infection models and one type of mouse urinary tract infection model. This work provides a new strategy to achieve antibacterial coatings with long-term activities for preventing implantable medical device associated infections.