Dopamine improves apple replant disease resistance by regulating physiological resilience and rhizosphere microbial community structure

作     者：Peihua Du Yang Cao Huaite Liu Jiahao Ji Wei Sun Xueying Zhang Jizhong Xu Bowen Liang Peihua Du;Yang Cao;Huaite Liu;Jiahao Ji;Wei Sun;Xueying Zhang;Jizhong Xu;Bowen Liang

作者机构：College of HorticultureHebei Agricultural UniversityBaoding 071001China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2024年第23卷第9期

页      面：3025-3044页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 090301[农学-土壤学] 

基　　金：supported by National Natural Science Foundation of China(31901964) the Science and Technology Project of Hebei Education Department,China(BJK2022012) the Innovation Ability Training Project for Graduate Student of Hebei Province,China(CXZZBS2023071) the Introduced Talents Project of Hebei Agricultural University,China(YJ201904) the Key Research and Development Project of Hebei Province,China(21326308D-02-03) the Earmarked Fund for the China Agricultural Research System,China(CARS-27) 

主　　题：continuous cropping barrier exogenous dopamine Malus hupehensis Rehd. 15N-labeling soil microbiome 

摘      要：Apple replant disease(ARD)is a complex agricultural problem caused by multiple stressors that can lead to increased reactive oxygen species(ROS)levels and limited nutrient utilization in ***,existing countermeasures cannot effectively address this ***,we used Malus hupehensis as a test organism to investigate whether the pleiotropic molecule dopamine can alleviate ARD using pot *** application of 100μmol L-1 dopamine significantly promoted the growth of apple seedlings in the replanted soil,with a relative growth rate increase of 17.44%.Our results revealed two major pathways by which dopamine regulates ARD resistance in apple ***,dopamine effectively reduces the level of ROS and activates the expression of genes related to nitrogen(N)transport and *** those genes,MdNLP5,MdNRT1.1,MdNLP2,MdNRT2.5,MdNLP3,MdNRT2.4,MdNADH-GAGOT,and MdFd-GAGOT were strongly regulated by *** regulatory effects promoted the uptake and utilization of soil N by the ***,dopamine improved the physical and chemical properties,enhanced microbial community diversity,and promoted mutual cooperation between microbial communities in the ***,dopamine altered the microbial structure of rhizosphere soil(upregulating Clostridiales,Gaiellales,Sordariales and Mortierellales;downregulating Micrococcales,Longimicrobiales,Hypocreales and Cystobasidiales).Notably,dopamine significantly upregulated the abundances of Gaiella and Mortierella,both of which were positively correlated with soil urease activity,soil available N content,plant growth and N *** also significantly downregulated the abundance of the plant pathogen Gibberella(by 11.71-fold)in replant *** results provide insights into the mechanisms by which dopamine promotes ARD resistance,and can promote the sustainable development of the apple industry.