Altered vasoactive intestinal peptides expression in irritable bowel syndrome patients and rats with trinitrobenzene sulfonic acid-induced colitis

作     者：Arseima Y Del Valle-Pinero LeeAnne B Sherwin Ethan M Anderson Robert M Caudle Wendy A Henderson 

作者机构：Digestive Disorders UnitBiobehavioral BranchDivision of Intramural ResearchNational Institute of Nursing ResearchNational Institutes of HealthDepartment of Health and Human ServicesBethesdaMD 20892United States Department of NeuroscienceUniversity of Florida College of MedicineGainesvilleFL 32610United States Department of Oral and Maxillofacial SurgeryUniversity of Florida College of DentistryGainesvilleFL 32610United States 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2015年第21卷第1期

页      面：155-163页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by National Institutes of Health grant,No.NS045614 the Ruth L.Kirschstein National Research Service Award,No.1F31 DK083165-01A1 from the National Institute of Diabetes and Digestive and Kidney Diseases Division of Intramural Research,National Institute of Nursing Research,No.1ZIANR000018-01-05 to Henderson WA and Intramural Training Award to Del Valle-Pinero AY and Sherwin LB 

主　　题：Irritable bowel syndrome Vasoactive intestinal pep 

摘      要：AIM: To investigate the vasoactive intestinal peptides(VIP) expression in irritable bowel syndrome(IBS) and trinitrobenzene sulfonic acid(TNBS) induced ***: The VIP gene expression and protein plasma levels were measured in adult participants(45.8% male) who met Rome Ⅲ criteria for IBS for longer than 6 mo and in a rat model of colitis as induced by *** and colons were collected from naive and inflamed *** assessing inflammation(i.e.,weight changes and myeloperoxidase levels) were assessed on days 2,7,14 and 28 and compared to *** hypersensitivity of the rats was assessed with colo-rectal distension and mechanical threshold testing on hind *** patients(n = 12) were age,gender,race,and BMI-matched with healthy controls(n = 12).Peripheral whole blood and plasma from fasting participants was collected and VIP plasma levels were assayed using a VIP peptide-enzyme *** gene expression of VIP was analyzed using a custom PCR ***: TNBS induced colitis in the rats was confirmed with weight loss(13.7 ± 3.2 g) and increased myeloperoxidase *** hypersensitivity tocolo-rectal distension was increased in TNBS treated rats up to 21 d and resolved by day *** hypersensitivity was also increased up to 14 d post TNBS induction of *** expression of an inflammatory marker myeloperoxidase was significantly elevated in the intracellular granules of neutrophils in rat models following TNBS treatment compared to naive *** confirmed the induction of inflammation in rats following TNBS *** plasma concentration was significantly increased in rats following TNBS treatment as compared to naive animals(P 0.05).Likewise,the VIP gene expression from peripheral whole blood was significantly upregulated by 2.91-fold in IBS patients when compared to controls(P 0.00001; 95%CI).VIP plasma protein was not significantly different when compared with controls(P = 0.193).CONCLUSION: Alterat