Microarray analysis of gene expression after electrical stimulation of the dura mater surrounding the superior sagittal sinus in conscious adult rats

作     者：Jiang Lei Dong Zhao Li Fengpeng Liu Ruozhuo Qiu Enchao Wang Xiaolin Yu Shengyuan 

作者机构：Department of Neurology Chinese People' s Liberation Army General Hospital Beijing 100853 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2014年第127卷第4期

页      面：734-741页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：grants from the National Natural Science Foundation of China 

主　　题：migraine electric stimulation superior sagittal sinus microarray analysis 

摘      要：Background The molecular and cellular origins of migraine headache are among the most complex problems in contemporary *** to now the pathogenesis of migraine still remains unclearly *** objective of this study was to explore new factors that may be related to the mechanism of *** The present study performed a comprehensive analysis of gene expression in the trigeminal nucleus caudalis induced by electrical stimulation of dura mater surrounding the superior sagittal sinus in conscious rats using microarray analysis followed by quantitative real-time reverse-transcribed polymerase chain reaction （qRT-PCR） ***＆#39;s two sample t-test was employed when two groups were compared.A P value 〈0.05 was considered to be statistically *** Comparing the placebo and the electrical stimulation groups,40 genes were determined to be significantly differentially *** significantly differentially expressed genes were involved in many pathways,including transporter activity,tryptophan metabolism,G protein signaling,kinase activity,actin binding,signal transducer activity,anion transport,protein folding,enzyme inhibitor activity,coenzyme metabolism,binding,ion transport,cell adhesion,metal ion transport,oxidoreductase activity,mitochondrion function,and *** of the genes were involved in more than 2 *** particular interest is the up-regulation of Phactr3 and Akap5 and the down-regulation of *** These findings may provide important clues for a better understanding of the molecular mechanism of migraine.