Sensitive method for rapid estimation of Lornoxicam in bulk and its dosage form by RP-HPLC
Sensitive method for rapid estimation of Lornoxicam in bulk and its dosage form by RP-HPLC作者机构:Institute of Research and Development Gujarat Forensic Sciences University A.R. College of Pharmacy and G.H.Patel Institute of Pharmacy
出 版 物:《Journal of Chinese Pharmaceutical Sciences》 (中国药学(英文版))
年 卷 期:2013年第22卷第1期
页 面:110-113页
核心收录:
学科分类:1007[医学-药学(可授医学、理学学位)] 100701[医学-药物化学] 10[医学]
摘 要:Lornoxicam (6-chloro-4-hydroxy-2-methyl-N-2-pyridyl-2H-thieno-[2,3-e]-1,2-thiazine-3-carboxamide-1,1-dioxide) is persist as a non-steroidal anti-inflammatory drug of the oxicam class with analgesic, anti-inflammatory and antipyretic properties. A fast, accurate and sensitive chromatographic method for estimation of Lornoxicam was developed as no official method available for detection. The chromatographic separation employs isocratic elution by utilizing an inertsil ODS-C 18 , 250 mm×4.6 mm, 5 μm columns. Mobile phase consisting of solvent (40 mL acetonitrile and 60 mL 0.1 M phosphate buffer (pH 6.8 was adjusted with triethylamine)) endowed at a flow rate of 1.0 mL/min. The analyte was detected and quantified at 290 nm using UV detector. The method was validated according to ICH guidelines, illustrating to be accurate (recovery 99.08%-101.13%) and precise (intraday (0.27-1.32) and interday (0.59-1.59))within the corresponding linear range (10-60 μg/mL) with r 2 0.9992.