Genetic changes of p53,K-ras,and microsatellite instability in gallbladder carcinoma in high-incidence areas of Japan and Hungary

作     者：Masayuki Nagahashi Yoichi Ajioka Istvan Lang Zoltan Szentirmay Miklos Kasler Hiroto Nakadaira Naoyuki Yokoyama Gen Watanabe Ken Nishikura Toshifumi Wakai Yoshio Shirai Katsuyoshi Hatakeyama Masaharu Yamamoto 

作者机构：Division of Molecular and Diagnostic Pathology Niigata University Graduate School of Medical and Dental Sciences Niigata 951-8510 Japan Department of Medical Oncology "B" National Institute of Oncology Budapest H-1122 Hungary Department of Molecular Pathology National Institute of Oncology Budapest H-1122 Hungary Department of Head and Neck Surgery National Institute of Oncology Budapest H-1122 Hungary Department of Nursing Faculty of Nursing Social Welfare and Psychology Niigata Seiryo University Niigata 951-8510 Japan Division of Digestive and General Surgery Niigata University Graduate School of Medical and Dental Sciences Niigata 951-8510 Japan Department of Community Preventive Medicine Niigata University Graduate School of Medical and Dental Sciences Niigata 951-8510 Japan 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2008年第14卷第1期

页      面：70-75页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Gallbladder Gallbladder Neoplasms K-ras Microsatellite instability p53 

摘      要：AIM： To disclose geographic differences in genetic changes involved in gallbladder carcinogenesis between two distinct high-incidence areas of Japan and Hungary. METHODS： We examined 42 cases of gallbladder carcinoma： 22 Japanese and 20 Hungarian cases, p53 mutations at exons 5 to 8 and K-ras mutations at codon 12 were tested by direct sequencing. Microsatellite instability was determined from fluorescent dye-labeled PCR amplifications of flve-microsatellite markers （BAT-25, BAT-26, D2S123, DSS346, and D17S250）. RESULTS： Mutations of p53 were detected in 11 of 22 Japanese cases and 6 of 18 Hungarian cases （11/22 vs 6/18, P = 0.348）. Transition at CpG sites was found in none of 11 Japanese cases and 2 of 6 Hungarian cases; the difference was marginally significant （0/11 vs 2/6,P = 0.110）. K-ras mutations were detected in only one of the Hungarian cases. Eight of 19 （42.1%） ]apanese cases were MSI-high （presence of novel peaks in more than one of the five loci analyzed）, whereas only 1 of 15 （6.7%） Hungarian cases was MSI-high （P = 0.047）. CONCLUSION： It appears that the p53 mutations and MSI differ in patients with gallbladder carcinoma between two distinct high-incidence areas. Geographic variation might exist in the process of gallbladder carcinogenesis.