Anti-HIV-1 Activities of 4 Telomerase Restrictors

作     者：YU Xin WANG Jinghui de Giuli Morghen C Radaelli A Zanotto C Beggio p 

作者机构：College of Pharmacy Wuhan University Wuhan 430072 Hubei China Medical School Huangshi Institute of Technology Huangshi 435003 Hubei China Laboratory of Molecular Virology Department of Medical Pharmacology University of Milan Via Vanvitelli 32 20129 Milano Italy 

出 版 物：《Wuhan University Journal of Natural Sciences》 (武汉大学学报（自然科学英文版）)

年 卷 期：2007年第12卷第6期

页      面：1113-1117页

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：Supported by China Scholarship Counsil(CSC) and Wuhan Chenguang Program of Wuhan City (2003002016-24) 

主　　题：telomerase telomerase restrictor HIV anti-HIV Agents Screening 

摘      要：MTT Cell Proliferation Assay was used to optimize the concentration of Telomerase Restrictors（TRs） with minimum toxicity to the selected cells. FACSort flow cytometer and Innotest P24 HIV（Human immtmodeficiency Virus） antigen mAb ELISA Kit were used to investigate the anti-HIV-1 activities of TRs. The results showed that TRs had low cytotoxicity to the PBMC （Peripheral Blood mononuclear cells） and CEM/GFP if the concentration of TRs was at 50μmol/L or below, and the supernatant from PBMC pretreated with SHIV and TR1-001 /TR1-002 could not infect the PBMC, while can infect the C8166 with reduced infectivity, which suggested that the TRs may be one of the novel resources for screening anti-HIV-1 agents.