Comparison of scanning electron microscopy findings regarding biofilm colonization with microbiological results in nasolacrimal stents for external, endoscopic and transcanalicular dacryocystorhinostomy

作     者：Melike Balikoglu-Yilmaz Tolga Yilmaz Sule Cetinel Umit Taskin Ayse Banu Esen Muhittin Taskapili Timur Kose 

作者机构：Department of Ophthalmology Bagcilar Education and Research Hospital Department of Histology & Embryology Marmara UniversityFaculty of Medicine Department of Otorhinolaryngology Bagcilar Education and Research Hospital1Istanbul 34200 Turkey Department of Microbiology and Clinical MicrobiologyBagcilar Education and Research Hospital Department of Biostatistics and Medical Informatics Ege University Faculty of Medicine 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2014年第7卷第3期

页      面：534-540页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by Institutional Review Board of Bagcilar Education and Research Hospital Istanbul Turkey(No.1852) 

主　　题：biofilms nasolacrimal duct obstruction epiphora dacryocystitis scanning electron microscopy 

摘      要：AIM:To compare bacterial biofilm colonization in lacrimal stents following external dacryocystorhinostomy(EX-DCR),endoscopic dacryocystorhinostomy(EN-DCR),and transcanalicular dacryocystorhinostomy(TC-DCR)with multidiode ***:This prospective study included 30consecutive patients with nasolacrimal duct obstruction who underwent EXT-,EN-,or *** removed lacrimal stent fragments and conjunctival samples were *** lacrimal stent biofilms were examined by scanning electron microscopy(SEM).RESULTS:Eleven(36.7%)of the 30 lacrimal stent cultures were positive for aerobic bacteria(most commonly Staphylococcus epidermidis and Pseudomonas aeruginosa).However anaerobic bacteria and fungi were not identified in the lacrimal stent ***-seven(90%)patients had biofilmpositive lacrimal *** conjunctival culture positivity after the DCR,biofilm positivity on stents,the grade of biofilm colonization,and the presence of mucus and coccoid and rod-shaped organisms did not significantly differ between any of the groups(P0.05).However,a significant difference was found when the SEM results were compared to the results of the lacrimal stent and conjunctival cultures(P0.001).CONCLUSION:Type of dacryocystorhinostomy(DCR)surgery did not affect the biofilm colonization of the lacrimal *** also appears to be more precise than microbiological culture for evaluating the presence of biofilms on lacrimal stents.