Production of transgenic mice carrying green fluorescence protein gene by a lentiviral vector-mediated approach

作     者：ZHANG Jingzhi, GUO Xinbing, XIE Shuyang, ZHU Yiwen, HUANG Ying, WANG Shu and REN Zhaorui (Shanghai Institute of Medical Genetics, Shanghai JiaoTong University, Shanghai 200040, China) 

作者机构：[a]Shanghai Institute of Medical Genetics Shanghai JiaoTong University Shanghai China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2006年第16卷第8期

页      面：827-832页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by the Major State Basic Research Development Program of China (Grant No. 2004CB518806) National Natural Science Foundation of China (Grant No. 30571777) 

主　　题：lentiviral vector transgene mouse integration GFP. 

摘      要：A pseudo-lentivirus, which carries green fluorescence protein (GFP) expressing cassette, was injected into the periv itelline space of murine fertilized oocytes before transplanting into the oviducts of the foster mothers. The GFP transgenic pups were then obtained. By PCR amplification, fluorescent microscopy and flow assisted cytometry sorting analysis, we found that the integration rate of the transgene was estimated at above 40% . Real-time PCR analysis indicated that the copy number of the integrated GFP cassette was around 40. Fluorescent in situ hybridization analysis demonstrated that the integration pattern was random but inheritable. The transgenic mice with multi-integration sites and various expression levels possessed a great value in practice as well as research. The approach reported herein provides an efficient way to generate and screen the transgenic mouse strains.