Effect of hydraulic retention time on the hydrogen yield and population of Clostridium in hydrogen fermentation of glucose

作     者：CHU Chunfeng EBIE Yoshitaka INAMORI Yuhei KONG Hainan 

作者机构：School of Environmental Science and Engineering Shanghai Jiao Tong University Shanghai 200240 China National Institute for Environmental Studies Tsukuba 305-8506 Japan Faculty of Symbiotic Systems Science Fukushima University Fukushima 960-1296 Japan 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2009年第21卷第4期

页      面：424-428页

核心收录：

学科分类：0710[理学-生物学] 083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 071007[理学-遗传学] 

主　　题：bio-hydrogen fluorescence in-situ hybridization genus Clostridium 

摘      要：The conversion of glucose to hydrogen was evaluated using continuous stirred tank reactor at pH 5.5 with various hydraulic retention times （HRT） at 30℃. Furthermore, the population dynamics of hydrogen-producing bacteria was surveyed by fluorescence in-situ hybridization using probe Clost IV targeting the genus Clostridium based on 16S rRNA. It was clear that positive correlation was observed between the cells quantified with probe Clost IV and hydrogen yield of the respective sludge. The numbers of hydrogen- producing bacteria were decreased gradually with increasing HRT, were 9.2 × 10^8, 8.2 × 10^8, 2.8 × 10^8, and 6.2 × 10^7 cell/mL at HRT 6, 8, 12, and 14 h, respectively. The hydrogen yield was 1.4-1.5 mol H2/mol glucose at the optimum HRT range 6-8 h. It is considered that the percentage of the hydrogen-producing bacteria to total bacteria is useful parameter for evaluation of hydrogen production process.