Effect of hepatic ischemia-reperfusion on bile canalicular F-actin microfilaments in rats

作     者：Yiming Li Hua Li Jidong Liu Hong Ji 

作者机构：Department of General Surgery the Second Affiliated Hospital of Xi' an Jiaotong University Xi' an 710004 China 

出 版 物：《Journal of Nanjing Medical University》 (南京医科大学学报（英文版）)

年 卷 期：2009年第23卷第2期

页      面：137-142页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by National Natural Science Foundation of China(30571765) 

主　　题：ischemia-reperfusion cholestasis F-actin 

摘      要：Objective：To investigate the effect of hepatic ischemia-reperfusion（I/R） on bile canalicular F-actin microfilaments in rats. Methods： A rat model of hepatic ischemia-reperfusion was employed and the ischemia time was 35 min. The activity of serum alanine aminotransferase （ALT）, aspartate aminotransferase（AST）, γ-glutamyl transferase（GGT） and the level of total bilirubin（TBIL） were measured. Changes in the bile canaliculi were observed by transmission electron microscope. The modification of F-actin microfilaments was quantified by using FITC-Phalloidin and analyzed by confocal laser scanning microscopy imaging. Results：Modifications of F-actin staining were consistent with the observations made by transmission electron microscopy. The staining of F-actin was normal in hepatocytes before reperfusion but decreased significantly after reperfusion, and there was a marked loss of canalicular microvilli after reperfusion, which coincided with abnormal serum GGT and TBIL levels. Conclusion：Reperfusion, not short-term ischemia, induced a disruption of F-actin microfilaments and a loss of microvilli. These modifications could lead to the impaired bile secretion by damaging canalicular contraction, and could be the main mechanisms of cholestasis after hepatic ischemia-reperfusion in rats.