Adenovirus-mediated HSV-TK Gene Therapy Using hTERT Promoter in CNE Cells in vitro

作     者：ZHANG Yu YU Xiang-hui ZHA Xiao KONG Wei 

作者机构：Vaccine Research Center College of Life Science Jilin University Changchun 130012 P. R. China Department of Radiotherapy Department of Molecular Pharmacology Sichuan Tumor Hospital Chengdu 610041 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2009年第25卷第1期

页      面：60-63页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 

基　　金：Supported in Part by the Agency of Science and Technology of Sichuan Province, China(No.2008JY0020) the National Natural Science Foundation of China(No.30872396). 

主　　题：hTERT HSV-TK Adenovirus Nasopharyngeal carcinoma 

摘      要：Human telomerase reverse transcriptase（hTERT） activity was detected in human nasopharyngeal carcinoma celI（CNE） but not in human normal lung fibroblas t（CCD-11Lu）. Recombinant adenoviruses Ad-CMV-TK-enh and Ad-hTERT-TK-enh were constructed and infected into normal fibroblasts and nasopharyngeal carcinoma cells. Ad-CMV-TK-enh with 100 μmol/L of ganciclovir（GCV） caused 87% of CCD-11Lu cells death and 91% of CNE cells death, Ad-hTERT-TK-enh with 100 μmol/L of GCV caused 24% of CCD-11Lu cells death and 79% of CNE cells death. These results indicate that the Ad-hTERT-TK-enh with GCV may be a useful method in suppressing tumor growth in targeted nasopharyngeal carcinoma gene therapy.