3D-printed aerogels as theranostic implants monitored by fluorescence bioimaging

作     者：Ana Iglesias-Mejuto Rui Pinto Pedro Faísca JoséCatarino João Rocha Luisa Durães Maria Manuela Gaspar Catarina Pinto Reis Carlos A.García-González 

作者机构：AerogelsLabI+D Farma Group(GI-1645)Department of PharmacologyPharmacy and Pharmaceutical TechnologyFaculty of PharmacyiMATUS and Health Research Institute of Santiago de Compostela(IDIS)Universidade de Santiago de CompostelaE-15782Santiago de CompostelaSpain Research Institute for Medicines(iMed.ULisboa)Faculty of PharmacyUniversidade de LisboaAv.Professor Gama Pinto1649-003LisboaPortugal Joaquim Chaves SaúdeJoaquim Chaves Laboratório de Análises ClínicasMiraflores1495069AlgésPortugal CECAV-Faculty of Veterinary Medicina-Lusófona University-Lisbon University CenterCampo Grande 3761749-024LisboaPortugal Faculty of Veterinary Medicina-Lusófona University-Lisbon University CenterCampo Grande 3761749-024LisboaPortugal University of CoimbraCERES-Chemical Engineering and Renewable Resources for SustainabilityDepartment of Chemical Engineering3030-790CoimbraPortugal Instituto de Biofísica e Engenharia Biomédica(IBEB)Faculdade de CiênciasUniversidade de LisboaCampo Grande1749-016LisboaPortugal 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2024年第41卷第11期

页      面：471-484页

核心收录：

学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 

基　　金：MICIU/AEI/10.13039/501100011033[Grants PID2020-120010RB-I00 and PDC2022-133526-I00] ERDF/EU and European Union NextGenerationEU/PRTR,Fundação para a Ciência e Tecnologia(FCT),doi:10.54499/UIDB/00645/2020,doi:10.54499/UIDP/00645/2020,UIDB/04138/2020,UIDP/04138/2020,doi:10.54499/UIDB/00102/2020 and doi:10.54499/UIDP/00102/2020 The work was carried out in the framework of the COST Innovators Grant IG18125“Technical,commercial and societal innovations on aerogels towards circular economy”(ECO-AERoGELS)funded by the European Commission.A.I.-M 

主　　题：Upconversion nanoparticles Aerogels Theranostic implants In vivo fluorescence 

摘      要：Aerogel scaffolds are nanostructured materials with beneficial properties for tissue engineering *** tracing of the state of the aerogels after their implantation is challenging due to their variable biodegradation rate and the lack of suitable strategies capable of in vivo monitoring the *** nanoparticles(UCNPs)have emerged as advanced tools for in vitro bioimaging because of their fluorescence *** this work,highly fluorescent UCNPs were loaded into aerogels to obtain theranostic implants for tissue engineering and bioimaging applications.3D-printed alginate-hydroxyapatite aerogels labeled with UCNPs were manufactured by 3D-printing and supercritical CO2 drying to generate personalize-to-patient *** physicochemical performance of the resulting structures was evaluated by printing fidelity measurements,nitrogen adsorption-desorption analysis,and different microscopies(confocal,transmission and scanning electron microscopies).Stability of the aerogels in terms of physicochemical properties was also tested after 3 years of *** was evaluated in vitro by different cell and hemocompatibility assays,in ovo and in vivo by safety and bioimaging studies using different murine *** profile,tissue index and histological evaluations of the main organs unveiled an in vivo downregulation of the inflammation after implantation of the ***-decorated aerogels were first-time manufactured and long-term traceable by fluorescence-based bioimaging until 3 weeks post-implantation,thereby endorsing their suitability as tissue engineering and theranostic nanodevices(*** implants).