The Regulatory Role and Mechanism of Circadian Rhythm in Hemoglobin Co-cultured Neurovascular Unit

作     者：Fang Xue Wenchao Chen Xia Lian Guanghui He Jingyuan Tian Yinghong Liu Gaiqing Wang Fang Xue;Wenchao Chen;Xia Lian;Guanghui He;Jingyuan Tian;Yinghong Liu;Gaiqing Wang

作者机构：Department of NeurologyThe Second Clinical School of MedicineShanxi Medical UniversityTaiyuan 030002ShanxiChina The Second Clinical School of MedicineShanxi Medical UniversityTaiyuan 030002ShanxiChina Department of Clinical labThe First People’s Hospital of ZhaotongZhaotong 657000YunnanChina Department of NeurologySanya Central Hospital(Hainan Third People’s Hospital)Hainan Medical UniversitySanya 57200HainanChina 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2024年第37卷第7期

页      面：726-738页

核心收录：

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.82160237) the Key Research and Development Program in Hainan Province(No.ZDYF2023SHFZ104) Natural Science Foundation of Hainan Province(No.822MS210) 

主　　题：Blood-brain barrier Circadian rhythm Neurovascular unit Melatonin receptor 1A Aquaporin-4 

摘      要：disruption of the blood-brain barrier(BBB),leading to vasogenic edema,plasma protein extravasation,and infiltration of neurotoxic *** clearance capacity of the brain plays a crucial role in maintaining BBB homeostasis and facilitating patient recovery after *** study aimed to investigate the effect of circadian rhythms on BBB function,neuronal damage,and clearance *** The transwell model and hemoglobin were co-cultured to simulate the BBB environment after *** intervention with different light groups,neuronal apoptosis was determined,glial phagocytosis was analyzed,the expression of endogenous clearing-related proteins aquaporin 4(AQP4)and low-density lipoprotein receptor-related protein 1(LRP1)was detected by western blotting and immunofluorescence dual standard method,and the expression of the tight junction protein occludin and melatonin receptor 1A(MTNR1A)was quantitatively *** Circadian rhythms play a key role in maintaining the integrity of the BBB,reducing oxidative stress-induced neuronal damage,and improving microglial ***,the expression of occludin and MTNR1A in neurovascular unit(NVU)co-cultured with hemoglobin improved the expression of AQP4 and LRP1,the key proteins in the NVU’s endogenous brain clearance *** Circadian rhythm(alternating black and white light)protects the NVU BBB function after ICH,promotes the expression of proteins related to the clearance of the hematoma,provides new evidence for the clinical treatment of patients recovering from ICH,and improves the circadian rhythm to promote brain metabolism and hematoma clearance.