Leech Poecilobdella manillensis protein extract ameliorated hyperuricemia by restoring gut microbiota dysregulation and affecting serum metabolites
作者机构：Medical CollegeGuangxi UniversityNanning 530004Guangxi Zhuang Autonomous RegionChina Department of Traditional Chinese MedicineHIV/AIDS Clinical Treatment Center of Guangxi(Nanning)The Fourth People’s Hospital of NanningNanning 530023Guangxi Zhuang Autonomous RegionChina Department of Science and TechnologyRuikang Hospital Affiliated to Guangxi University of Chinese MedicineNanning 530011Guangxi Zhuang Autonomous RegionChina General Manager’s OfficeGuangxi Fuxinyi Biological Technology Co.Ltd.Pingnan 537300Guangxi Zhuang Autonomous RegionChina Department of Joint and Sports MedicineRuikang Hospital Affiliated to Guangxi University of Chinese MedicineNanning 530011Guangxi Zhuang Autonomous RegionChina Department of GerontologyNanning Social Welfare HospitalNanning 530004Guangxi Zhuang Autonomous RegionChina
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2024年第30卷第29期
页 面：3488-3510页
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Supported by National Natural Science Foundation of China No.82160843
主 题：Gut microbiota Metabolism Multi-omics Poecilobdella manillensis Sphingolipid metabolism pathway Galactose metabolism pathway Hyperuricemia
摘 要：BACKGROUND Hyperuricemia(HUA)is a public health concern that needs to be solved *** lyophilized powder of Poecilobdella manillensis has been shown to significantly alleviate HUA;however,its underlying metabolic regulation remains *** To explore the underlying mechanisms of Poecilobdella manillensis in HUA based on modulation of the gut microbiota and host *** A mouse model of rapid HUA was established using a high-purine diet and potassium oxonate *** mice received oral drugs or ***,16S rRNA sequencing and ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry-based untargeted metabolomics were performed to identify changes in the microbiome and host metabolome,*** levels of uric acid transporters and epithelial tight junction proteins in the renal and intestinal tissues were analyzed using an enzyme-linked immunosorbent *** The protein extract of Poecilobdella manillensis lyophilized powder(49 mg/kg)showed an enhanced anti-trioxypurine ability than that of allopurinol(5 mg/kg)(P0.05).A total of nine bacterial genera were identified to be closely related to the anti-trioxypurine activity of Poecilobdella manillensis powder,which included the genera of Prevotella,Delftia,Dialister,Akkermansia,Lactococcus,Escherichia_Shigella,Enterococcus,and ***,22 metabolites in the serum were found to be closely related to the anti-trioxypurine activity of Poecilobdella manillensis powder,which correlated to the Kyoto Encyclopedia of Genes and Genomes pathways of cysteine and methionine metabolism,sphingolipid metabolism,galactose metabolism,and phenylalanine,tyrosine,and tryptophan *** analysis found that changes in the gut microbiota were significantly related to these *** The proteins in Poecilobdella manillensis powder were effective for ***,they are associated with improvements in g
维普期刊数据库