A Comparative Analysis of Culture Media for Optimizing the Mycelial Growth and Sporulation of Stemphylium vesicarium Cause of White Blotch of Onion
A Comparative Analysis of Culture Media for Optimizing the Mycelial Growth and Sporulation of Stemphylium vesicarium Cause of White Blotch of Onion作者机构：Department of Plant Pathology Sher-e-Bangla Agricultural University Dhaka 1207 Bangladesh Department of Botany University of Dhaka Dhaka 1000 Bangladesh
出 版 物：《Journal of Agricultural Science and Technology(A)》 (农业科学与技术（A）)
年 卷 期：2015年第5卷第6期
页 面：440-448页
学科分类：09[农学]
主 题：Onion white blotch S. vesicarium V-7 juice agar.
摘 要：This study was conducted to identify a cheap and suitable culture medium for the mycelial growth and sporulation of Sternphylium vesicarium and to determine the cultural and morphological variability of this pathogen. A total of 24 isolates of S. vesicarium collected from eight different onion growing areas were characterized in terms of cultural and morphological aspects. Front colony colors were greenish brown to dirty white, deep grey to whitish, light grey to whitish, deep greenish white, light grey and dirty white to greenish. Reverse colony colors were brown, deep brown and light brown. Colony shapes were circular and irregular with umbonate, raised and flat type colony elevation. Colony textures were cottony, fluffy and velvety with entire, undulate and filiform type colony margin. Among the culture media, V-7 juice agar found to be the most suitable culture media for mycclial growth of S. vesicarium. The sporulation of the fungus was remarkably influenced by V-7 juice mixed with potato dextrose agar （PDA） media, this media exhibited the highest sporulation （87.76-169.0/mm^2） of S. vesicariumin comparison with other media. The minimum days （28 d to 31 d） for conidial production were observed on V-7 juice agar medium. The length of conidia varied from 14.6 μm to 30.6 μm. The maximum mean length of conidia was 29.97 μm found in isolate DSSA, while the minimum mean length 17.36 μm was found in isolate MSMM 02. The breadth of conidia ranged from 4.7 μm to 15.7μm. The maximum mean breadth of conidia was 12.55 μm found in the isolate DSSA, while the minimum mean breadth 9.760 μm was found in the isolate CCKH 02. The horizontal septation varied from l to 3 and the longitudinal septation varied from 0 to 4.
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