ISOLATION OF PLASMID FROM THE BLUE-GREEN ALGA SPIRULINA PLATENSIS

作     者：秦松 童顺 张培军 曾呈奎 

作者机构：Experimental Marine Biology Laboratory Institute of Oceanology Academia Snica Qingdao 266071 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：1993年第11卷第3期

页      面：285-288页

核心收录：

学科分类：07[理学] 

基　　金：This research was supported in part by The President of The Chinese Academy of Sciences Foundation 

主　　题：plasmid blue-green alga Spindina platensis 

摘      要：CCC plasmid was isolated from an economically important blue-green alga- Spirulina platensis (1.7×106 dalton from the S6 strain and 1.2×106 dalton from the F, strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference in the molecular weight of the OOC DNAs from the two strains differing in form suggests that plasmid may be related with the differentiation of algal form. This modified method, which does not use any lysozyme, is a quick and effective method of plasmid isolation, especially for filamentous blue-green algae.