DETECTION OF EB VIRUS EARLY ANTIGEN IN NASOPHARYNGEAL CARCINOMA (NPC) USING A NEW MONOCLONAL ANTIBODY--BAE-5

作     者：张昌卿 肖锡宾 冯凯涛 孙韵 张锋 宗永生 

作者机构：Tumor Hospital Sun Yat-sen University of Medical Sciences Guanzhou 510060 Department of Pathology Sun Yat-sen University of Medical Sciences Guanzhou 510060 mouse monoclonal antibody against EB virus EA-R named BAE- 5 was prepared applying hybridoma technique by use of n-butyric add and croton oil induced Raji cells as immunogen.And then 34 NPCs and 29 mm-NPC neoplasms were detected by the BAE-5 using APAAP immunohistochemical staining method.The results Indicated that all of the 34 NPCs and 9 of the 29 non-NPC neoplasms could reacted with the BAE-5 in spite of the columnar epithelium reserve cells showing positivity also. It is reasonable to come to the conclusion that most of the NPC cells can not only harbour EBV DNA but some of them be able to express EA-R. Some non-NPC neoplasms being demonstrated BAE-5 posttlvlty may be due to the presence of EBV EA- R antigenic epitope or polypeptides simulating the structure of EA-R. The authors think that these findings have theoretical significance for understanding the role of EBV- encoded poiypeptides especially EA complex In the development and progression of NPC as well as some non- NPC neoplasms harbouring EBV-DNA. 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：1993年第5卷第1期

页      面：38-45页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：monoclonal antibody,immunohistochemistryNasopharyngeal carcinoma EB virus, 

摘      要：A mouse monoclonal antibody against EB virus EA-R named BAE- 5 was prepared applying hybridoma technique by use of n-butyric add and croton oil induced Raji cells as *** then, 34 NPCs and 29 mm-NPC neoplasms were detected by the BAE-5 using APAAP immunohistochemical staining *** results Indicated that all of the 34 NPCs and 9 of the 29 non-NPC neoplasms could reacted with the BAE-5 in spite of the columnar epithelium reserve cells showing positivity also. It is reasonable to come to the conclusion that most of the NPC cells can not only harbour EBV DNA but some of them be able to express EA-R. Some non-NPC neoplasms being demonstrated BAE-5 posttlvlty may be due to the presence of EBV EA- R antigenic epitope or polypeptides simulating the structure of EA-R. The authors think that these findings have theoretical significance for understanding the role of EBV- encoded poiypeptides, especially EA complex, In the development and progression of NPC as well as some non- NPC neoplasms harbouring EBV-DNA.