Studies on Synthesis of Stationary Phase Containing Nicotinamide Adenine Dinucleotide(NAD) Bonded to Phospholipid-Coated Aminated Silica for HPLC

作     者：Jin Mao YOU Zong Qin RUAN Jing Wu KANG (Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, 730000) 

作者机构：Lanzhou Inst. of Chemical Physics Chinese Academy of Sciences 730000 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：1997年第8卷第3期

页      面：243-246页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

主　　题：NAD Bonded to Phospholipid-Coated Aminated Silica for HPLC Studies on Synthesis of Stationary Phase Containing Nicotinamide Adenine Dinucleotide 

摘      要：Recently biospecific affinity chromatography has been widely used for the separation and purification of various enzymes and nucleic acids. In this paper, a series of synthetic reactions of solid-liquid phase were carried out on silica surface, using a macroporous(30 mu m), microspherical silica (8 mu m) as the matrix and gamma-aminopropyltriethoxysilane as the activating agent, the nicotinamide adenine dinucleotide(NAD) was bonded through its amino groups to the carboxylic groups of linked phospholipid which was bonded covalently on aminated support. The bonded stationary phase has high thermal stability, and could be used to separate of nucleotides with good resolution.