Inducible Expression of Translation Elongation Factor 1A Gene in Rice Seedlings in Response to Environmental Stresses 

作     者：李子银 陈受宜 

作者机构：中国科学院遗传研究所植物生物技术开放实验室北京100101 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：1999年第41卷第8期

页      面：800-806页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

主　　题：Rice Differential display PCR Translation elongation factor 1A Environmental factors Differential expression 

摘      要：Differences of gene expression between salinity_stressed and control rice ( Oryza sativa L. ssp. indica ) cultivar “Zhaiyeqing 8 were compared using differential display PCR (DD_PCR) technique. Sequence analysis of one salt_inducible cDNA clone revealed that this clone represented a new member of rice translation elongation factor 1A (eEF1A) gene family and was tentatively named REF1A. Northern blot hybridization using REF1A fragment as a probe was performed to investigate the expression of rice translation elongation factor 1A gene in response to various environmental factors. It was observed that expression of the eEF1A gene in rice shoots was dramatically induced by salinity stress or exogenous application of abscisic acid (ABA). The induction of this gene by ABA stress occurred more quickly than that by salinity stress. In addition, expression of rice translation elongation factor 1A gene was also induced by drought (15% PEG6000), cold (4 ℃) or heat_shock (37 ℃) stresses. The results suggested that the induction of translation elongation factor 1A gene expression by environmental stresses might reflect the general adaptive response of rice plants to the adverse circumstances.