Investigation of Function of Novel Sperm Binding Protein HBRP in Human

作     者：Jie ZHANG Ying LIU Wen-hui SU Guo-li WANG Zhi-hong ZONG Bing-zhi YU 

作者机构：Department of Biochemical and Molecular Biology China Medical University Shenyang 110001 China 

出 版 物：《Journal of Reproduction and Contraception》 (生殖与避孕（英文版）)

年 卷 期：2007年第18卷第3期

页      面：173-180页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：This research was supported by funds from the National Natural Science Foundation (30170987) of China 

主　　题：HBRP eukaryotic expression PKC activity 

摘      要：Objective To investigate the biology function of novel protein related to bovine seminal plasma protein in human testis. Methods Recombination pcDNA3/HBRP was constructed and transfected to HEK293 cell and permanently expression cell line was established. The activity of protein kinase C （PKC） of the cell line was detected by autoradiography method. Results The stable expression cell line of HBRP was obtained. The HBRP inhibited the activity of PKC significantly. Conclusion One of the new functions of novel sperm binding protein in human is the inhibitor action on activity of PKC. It may be involved in the sperm capacitation and acrosome reaction.