系统性硬皮病中自然杀伤细胞功能异常:细胞因子产物改变和杀伤活性缺陷

作     者：Horikawa M. Hasegawa M. Komura K. S. Sato 任建文 

作者机构：Department of DermatologyNagasakiUniversityGraduate School of Biomedical Sciences 1- 7- 1 Sakamoto Nagasaki 852- 8501 Japan Dr. 

出 版 物：《世界核心医学期刊文摘（皮肤病学分册）》 (Digest of the World Core Medical JOurnals:Dermatology)

年 卷 期：2006年第2卷第8期

页      面：14-14页

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 100102[医学-免疫学] 10[医学] 

主　　题：系统性硬皮病 自然杀伤细胞 细胞因子产物 细胞功能异常 自身免疫性结缔组织病 活性缺陷 NK细胞 免疫效应细胞 免疫调节因子 γ干扰素 

摘      要：Natural killer (NK) cells are innate immune effectors that produce various immunoregulatory cytokines. Recent studies have shown that NK cells are involved in the initiation of autoimmunity. In this study, we determined abnormalities of NK cells in systemic sclerosis (SSc), an autoimmune connective tissue disease, by assessing the frequency and absolute number, activation marker expression, cytokine production, and killing activity. The frequency and absolute number of NK cells increased in diffuse cutaneous SSc (dcSSc), whereas they were normal in limited cutaneous SSc (IcSSc). NK cells from both dcSSc and IcSSc patients exhibited activated phenotypes characterized by up-regulated CD16 and CD69 expression and downregulated CD62L expression. Interferon (IFN)-γ production by non-stimulated NK cells from both dcSSc and IcSSc patientswas increased compared to the normal control, whereas on stimulation, a reduced amount of IFN-γ was produced. Interleukin (IL)- 5 and IL- 10 production by non- stimulated NK cells and IL- 6 production by stimulated NK cells were augmented in dcSSc patients, but not in IcSSc patients. Despite the augmented cytokine production by non-stimulated NK cells, natural cytotoxicity activity and granzyme B secretion was reduced in NK cells from dcSSc and IcSSc patients. These results suggested that altered NK cell function contributes to immunological abnormalities in SSc.