Probiotic yeasts: Anti-inflammatory potential of various non-pathogenic strains in experimental colitis in mice

作     者：Benot Foligné Jo■lle Dewulf Pascal Vandekerckove Georges Pignède Bruno Pot 

作者机构：Institut Pasteur of LilleLactic acid Bacteria & Mucosal ImmunityCenter for Infection and Immunity of Lille1 rue du Pr CalmetteBP 245F-59019 LilleFrance University of Lille Nord de FranceF-59000 LilleFrance Centre National de la Recherche ScientifiqueUMR 8204F-59021 LilleFrance Institut National de la Santé et de la Recherche MédicaleU1019F-59019 LilleFrance Lesaffre InternationalDirection of Research and Developpement147 rue Gabriel PériBP 602759700 Marcq-en-BaroeulFrance 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2010年第16卷第17期

页      面：2134-2145页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Lesaffre group 

主　　题：Yeast Probiotics Strain specificity Experimental colitis 2-4-6-trinitrobenzene sulfonic acid 

摘      要：AIM: To evaluate the in vitro immunomodulation capacity of various non-pathogenic yeast strains and to investigate the ability of some of these food grade yeasts to prevent experimental colitis in ***: In vitro immunomodulation was assessed by measuring cytokines [interleukin (IL)-12p70,IL-10,tumor necrosis factor and interferon γ] released by human peripheral blood mononuclear cells after 24 h stimulation with 6 live yeast strains (Saccharomyces ssp.) and with bacterial reference strains.A murine model of acute 2-4-6-trinitrobenzene sulfonic acid (TNBS)-colitis was next used to evaluate the distinct prophylactic protective capacities of three yeast strains compared with the performance of prednisolone ***: The six yeast strains all showed similar non-discriminating anti-inflammatory potential when tested on immunocompetent cells in vitro .However,although they exhibited similar colonization patterns in vivo ,some yeast strains showed significant anti-inflammatory activities in the TNBS-induced colitis model,whereas others had weaker or no preventive effect at all,as evidenced by colitis markers (body-weight loss,macroscopic and histological scores,myeloperoxidase activities and blood inflammatory markers).CONCLUSION: A careful selection of strains is required among the biodiversity of yeasts for specific clinical studies,including applications in inflammatory bowel disease and other therapeutic uses.