Metabolic engineering and genome editing strategies for enhanced lipid production in microalgae

作     者：ANJANI DEVI CHINTAGUNTA SAMUDRALA PRASHANT JEEVAN KUMAR NUNE SATYA SAMPATH KUMAR 

作者机构：Department of BiotechnologyVignan’s Foundation ScienceTechnology and ResearchVadlamudiAndhra Pradesh522213India ICAR-Directorate of Floricultural ResearchPune412307India 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2024年第48卷第8期

页      面：1181-1195页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：partially supported by Department of Science and Technology Science and Engineering Research Board under Teachers Associateship for Research Excellence(TARE)Scheme(File Number TAR/2023/000036) 

主　　题：Oleaginous microalgae Biodiesel TAG synthesis Metabolic engineering Genome editing 

摘      要：Depleting global petroleum reserves and skyrocketing prices coupled with succinct supply have been a grave concern,which needs alternative sources to conventional *** microalgae have been explored for enhanced lipid production,leading towards biodiesel *** microalgae have short life cycles,require less labor,and space,and are easy to scale ***,the primary source of lipids needed to produce biodiesel,is accumulated by most *** article focuses on different types of oleaginous microalgae,which can be used as a feedstock to produce *** biosynthesis in microalgae occurs through fatty acid synthesis and TAG synthesis ***-depth discussions are held regarding other efficient methods for enhancing fatty acid and TAG synthesis,regulating TAG biosynthesis bypass methods,blocking competing pathways,multigene approach,and genome *** most potential targets for gene transformation are hypothesized to be a malic enzyme and diacylglycerol acyltransferase while lowering phosphoenolpyruvate carboxylase activity is reported to be advantageous for lipid synthesis.