Effect of WNT5A on epithelial-mesenchymal transition and its correlation with tumor invasion and metastasis in nasopharyngeal carcinoma

作     者：Hong-Hai Zhu Xiao-Yuan Zhu Ming-Hui Zhou Gen-Yang Cheng Wei-Hua Lou 

作者机构：Rhinology of the First Affiliated Hospital of Zhengzhou University Department of Otolaryngology-Head and Neck Surgerythe First Affiliated Hospital of Zhengzhou University Department of Nephrology of the First Affiliated Hospital of Zhengzhou University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2014年第7卷第6期

页      面：488-491页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：WNT5A Epithelial-mesenchymal transition Matrix metalloproteinase-2 E-cadherin Nasopharyngeal cancer Metastasis 

摘      要：Objective:To investigate the correlation between nasopharyngeal carcinoma cell WNT5A and epithelial-mesenchytnal transition(emt)/metastasis,and investigate its possible ***:RT-PCR and gene transfection were used to detect the expression of nasopharyngeal carcinoma cell strains WNT5A and EMT related factor *** transfcction of NPC cell line 5-8F was determined by liposome of plasmid with WNT5A *** differential expressions of WNTSA and EMT-related factors in cells before and after transfection were detected by *** scratch assay and Transwell assay were used to detect the motility abilities of cells before and after 5-8F ***:The expressions of WNT5A and EMT related factors matrix metalloproleinasc-2 of the WNTSA transferred group in the nasopharyngeal carcinoma cell line 5-8F were higher than the blank control group and the empty vector transferred group,and the transfer ability of the WNTSA transferred group was higher than that in the blank control group and the empty vector transferred group,while the expressions of EMT related factors E-cadherin were lower than that in the blank control group and the empty vector transferred group,and the transfer ability of the WNT5A transferred group was higher than that in the blank control group and the empty vector transferred ***:In nasopharyngeal carcinoma cells,WNT5A can regulate the epithelial-mesenchymal transition and affect the ability of tumor invasion and metastasis of nasopharyngeal carcinoma.