Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils,Lissorhoptrus oryzophilus Kuschel(Coleoptera:Curculionidae)
Differential gene expression profiling in the developed ovaries between the parthenogenetic and bisexual female rice water weevils,Lissorhoptrus oryzophilus Kuschel(Coleoptera:Curculionidae)作者机构:State Key Laboratory of Rice Biology Key Laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture Institute of Insect Sciences Zhejiang University Hangzhou 310029 China Department of Plant Protection College of Biosafety Science and Technology Hunan Agricultural University Changsha 410128 China Texas A & M University Agricultural Research and Extension Center 1509 Aggie Dr. Beaumont TX 77713 USA
出 版 物:《Chinese Science Bulletin》 (中国科学通报)
年 卷 期:2009年第54卷第20期
页 面:3822-3829页
核心收录:
学科分类:0710[理学-生物学] 07[理学] 09[农学] 0901[农学-作物学]
基 金:Supported by the National Key Basic Research and Development Program of China (Grant No. 2009CB119200) National High Technology Research and Development Program of China (Grant No.2007AA10Z220) Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (Grant No.2006BAD17B06)
主 题:基因差异表达 稻水象甲 发达国家 象虫科 鞘翅目 卵巢 女性 抑制性消减杂交
摘 要:The rice water weevil,Lissorhoptrus oryzophilus Kuschel(Coleoptera:Curculionidae),reproduces by sex in the Southeastern United States,but reproduces by parthenogenesis in California and other invaded regions in Asia and *** objective of this study was to create a parthenogenetic gene expression profile of the rice water weevil in order to gain a better insight into the molecular mechanisms of parthenogenesis in the *** subtractive hybridization(SSH) technique was employed for profiling differential gene expression in the developed ovary between the parthenogenetic and bisexual female rice water weevils.A total of 70 contigs were obtained,and the BLASTX search identified putatively 28 genes with differential *** to the cytological process of parthenogenesis,the tubulin alpha-1 chain and signal transduction genes *** selected for real time quantitative RT-PCR analyses,and their possible functions related to the molecular mechanism of parthenogenesis were *** tubulin alpha-1 chain and some signal transduction genes may be related to the molecular mechanisms of parthenogenesis of the rice water weevil.