Morphological and cytological assessments reveal pollen degradation causes pollen abortion in cotton cytoplasmic male sterility lines

作     者：PEI Qingyu LIU Jinshan GUO Chunping MA Xiaomei LIU Xiaoyan YOU Chunyuan LIN Hairong LI Zhibo ZHAO Ruihai ZHU Bo WU Yuanlong PAN Zhenyuan NIE Xinhui PEI Qingyu;LIU Jinshan;GUO Chunping;MA Xiaomei;LIU Xiaoyan;YOU Chunyuan;LIN Hairong;LI Zhibo;ZHAO Ruihai;ZHU Bo;WU Yuanlong;PAN Zhenyuan;NIE Xinhui

作者机构：Key Laboratory of Oasis Ecology Agricultural of Xinjiang Production and Construction CorpsAgricultural CollegeShihezi UniversityShihezi 832003XinjiangChina Seed Management Station of Xinjiang Production and Construction CorpsUrumqi 830000XinjiangChina Cotton InstituteXinjiang Academy of Agricultural and Reclamation ScienceShihezi 832000XinjiangChina Xinjiang Jinbo Seed Industry Co.LtdBoleXinjiang 833400China National Key Laboratory of Crop Genetic ImprovementCollege of Plant Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina Cotton Research InstituteShihezi Academy of Agriculture ScienceShihezi 832011XinjiangChina Agricultural Science Research Institute of the 5th Division of Xinjiang Production and Construction CorpsShuanghe 833408XinjiangChina 

出 版 物：《Journal of Cotton Research》 (棉花研究（英文）)

年 卷 期：2024年第7卷第3期

页      面：266-276页

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the Fund for the Biological Breeding-Major Projects in National Science and Technology(2023ZD04038) the Key Project for Agricultural Breakthrough in Core Technology of Xinjiang Production and Construction Crops(NYHXGG,2023AA102) the Key Project for Science and Technology Development of Shihezi city,Xinjiang Production and Construction Crops(2022NY01) 

主　　题：Cotton Cytoplasmic male sterility Genetic analysis Morphological characteristics Pollen development 

摘      要：Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm ***,only a few cytoplasmic male sterility(CMS)lines of cotton have been produced due to several challenges,like inadequate variation of agronomic traits,incomplete sterility,weak resilience of restorer lines,and difficulty in combining strong ***,the morphological and cytological identification of CMS in cotton will facilitate hybrid *** Two F_(2) segregating populations of cotton were constructed from cytoplasmic male sterile lines(HaA and 01A,maternal)and restorer lines(HaR and 26R,paternal).Genetic analysis of these populations revealed a segregation ratio of 3:1 for fertile to sterile *** analysis indicated no significant differences in traits of flower bud development between sterile and fertile ***,sterile plants exhibited smaller floral organs,shortened filament lengths,and anther atrophy on the flowering day in comparison with the fertile *** performed scanning electron microscopy(SEM),the two F_(2) populations revealed morphological variations in the anther *** analysis showed no significant differences in pollen development before pollen ***,between the pollen maturation and flowering stages,the tapetum layer of sterile plants degenerated prematurely,resulting in abnormal pollen grains and gradual pollen *** The results of this study suggest that fertility-restoring genes are controlled by a single dominant *** plants exhibit distinctive floral morphology,which is characterized by stamen atrophy and abnormal *** abortion occurs between pollen maturity and flowering,indicating that premature tapetum degradation may be the primary cause of pollen ***,our study provides a theoretical basis for utilizing CMS in hybrid breeding and in-depth investigation of the dominant conf