Highly sensitive potentiometric immunosensor for hepatitis B surface antigen diagnosis

作     者：YUAN Ruo TANG Dianping CHAI Yaqin ZHANG Lingyan LIU Yan ZHONG Xia DAI Jianyuan 

作者机构：Chong Qing Key Laboratory of Analytical ChemistryCollege of Chemistry and Chemical EngineeringSouthwest China Normal UniversityChongqing 400715China 

出 版 物：《Science China Chemistry》 (中国科学(化学英文版))

年 卷 期：2005年第48卷第1期

页      面：49-57页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：the National Natural Science Foundation of China(Grant No.29705001) the Chinese Education Ministry Foundation for Excellent Young Teachers the Natural Science Foundation of Chongqing City,China 

主　　题：nafion polyvinyl butyral colloidal Au hepatitis B potentiometric immunosensor platinum electrode. 

摘      要：A highly sensitive potentiometric immunosensor for the diagnoses of epidemic dis-eases has been developed by means of self-assembly to immobilize hepatitis B surface antibody(HBsAb)for the detection of hepatitis B surface antigen(HBsAg)as a *** first,the Nafion containing-SO^(-)_(3)groups was immobilized on a platinum electrode surface to absorb the-NH^(+)_(3)groups of antibody molecules via the opposite-charged adsorption technique,in the meantime,hepatitis B surface antibodies were adsorbed onto the surface of Au nanoparticles,then hepatitis B surface antibodies and Au nanopartilces were entrapped into polyvinyl butyral on the surface of Nafion *** modified procedure was further characterized by electrochemical impedance spectroscopy(EIS)and cyclic voltammetry(CV).The influence and factors influencing the per-formance of resulting immunosensor were studied in *** resulting immunosensor exhib-ited sigmoid curve with log HBsAg concentrations,high sensitivity,wide linear range from 26 to 1280 ng·mL^(-1)with a detection limit of 3.1 ng·mL^(-1),rapid potentiometric response(4 months).Analytical results of clinical samples show that the developed immunoassay is comparable with the enzyme-linked immunosorbent assays(ELISAs)method,implying a promising alternative approach for detecting HBsAg in the clinical diagnosis.