差异蛋白组学方法识别热休克蛋白27作为动脉粥样硬化的潜在标志物

作     者：Martin-Ventura J.L. Duran M.C. Blanco-Colio L.M. J. Egido 王亭忠 

作者机构：Vascular Research Laboratory Fundacion Jimenez Diaz Avenida Reyes Cat olicos 2 28040 Madrid Spain Dr. 

出 版 物：《世界核心医学期刊文摘（心脏病学分册）》 (Digest of the World Core Medical Journals(Cardiology))

年 卷 期：2005年第1卷第3期

页      面：30-30页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：差异蛋白组学 血管细胞 颈动脉斑块 分泌型蛋白 免疫组化法 颈动脉硬化 旋切 电泳分析 队列研究 质中 

摘      要：Background-We hypothesized that normal and pathological vessel walls display a differential pattern of secreted proteins. We have recently set up the conditions for comparing secretomes from carotid atherosclerotic plaques and control arteries using a proteomic approach to assess whether differentially secreted proteins could represent markers for atherosclerosis. Methods andResults-Normal endartery segments and different regions of endarterectomy pieces (noncomplicated/ complicated plaques) were incubated in protein-free medium, and the released proteinswere analyzed by 2D electrophoresis(2-DE). Among the differently secreted proteins, we have identified heat shock protein-27(HSP27). Surprisingly, compared with control arteries, HSP27 release was drastically decreased in atherosclerotic plaques and barely detectable in complicated plaque supernatants. HSP27 was expressed primarily by intact vascular cells of normal arteries and carotid plaques (immunohistochemistry). Plasma detection of soluble HSP27 showed that circulating HSP27 levels are significantly decreased in the blood of patients with carotid stenosis relative to healthy subjects(0.19 [0.1 to 1.95] versus 83[71.8 to 87.8]) ng/mL, P 0.0001). Conclusions-HSP27 secretion is decreased in complicated atherosclerotic plaques, and sHSP27 plasma levels are decreased in atherosclerotic patients compared with healthy subjects. Plasma sHSP27 levels could be a potential index of atherosclerosis, although further validation is needed in large patient cohorts.