Inhibition of Embryonic Stem Cell Differentiation by Gold Nanoparticles
Inhibition of Embryonic Stem Cell Differentiation by Gold Nanoparticles作者机构：School of Health Sciences Oakland University Rochester 48309 USA Oakland University-William Beaumont Institute for Stem Cell and Regenerative Medicine Oakland University Rochester 48309USA Department of Biological Sciences Oakland University Rochester 48309 USA
出 版 物：《Journal of Pharmacy and Pharmacology》 (药剂与药理学（英文版）)
年 卷 期：2014年第2卷第4期
页 面：243-256页
学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学]
基 金：Acknowledgments This research was supported by the OU-WB (Oakland University-William Beaumont) Interdisciplinary Research Program Oakland University Michigan Research Excellence Fund and the SIBHI (Summer Institute of Bioengineering and Health Informatics) which was funded by the National Institutes of Health and the National Science Foundation. We are thankful for Ms. Loan Dong and Dr. Harvey Qu for TEM studies and statistical analysis respectively
主 题：Nanomaterials embryotoxicity pluripotency self-renewal Oct 4
摘 要：Nanomaterials are attractive for use in technological advancements because of their small size and unique properties. As a result, there has been a rapid increase in the production and applications of nanomaterials. Nanoparticles like carbon, cadmium and silver are highly toxic and are known to cause oxidative stress. However, there are conflicting reports regarding the toxicity of gold nanoparticles. We have investigated the effects of gold nanoparticles on the growth and differentiation of ESCs （embryonic stem cells）. Analysis of ESCs treated with gold nanoparticles revealed a biphasic growth response. Higher concentrations （〉 20 ~tg/mL） of gold nanoparticles inhibited growth, whereas the lower concentrations （〈 10 lag/mL） stimulated ESC proliferation. Interestingly, ESC pluripotency was not affected by gold nanoparticles as demonstrated by the near normal expression of the specific pluripotent marker, Oct 4, and their differentiation potential. Inhibition of differentiation of both ESCs and embryoid bodies by gold nanoparticles suggest that they may pose developmental risks. Further analysis by transmission electron microscopy and atomic absorption spectroscopy revealed that gold nanoparticles were actively taken up by ESCs in a concentration dependent manner. These observations suggest that exposure to gold nanoparticles may cause embryotoxicity or effect early childhood development.
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